Genetic Analysis and Molecular Mapping of Novel White Striped Leaf Mutant Gene in Rice

doi:10.1016/S1672-6308(13)60142-0

Abstract

Abstract:

A new white striped leaf mutant wsl1 was discovered from Nipponbare mutated by ethyl methanesulfonate. The mutant showed white striped leaves at the seedling stage and the leaves gradually turned green after the tillering stage. The chlorophyll content of wsl1 was significantly lower than that of wild-type during the fourth leaf stage, tillering stage and booting stage. The numbers of chloroplast, grana and grana lamella were reduced and the thylakoids were degenerated in wsl1 compared with wild type. Genetic analysis showed that the wsl1 was controlled by a single recessive gene. Molecular mapping of the wsl1 was performed using an F2 population derived from wsl1/Nanjing 11. The wsl1 was finally mapped on the telomere region of chromosome 9 and positioned between simple sequence repeat markers RM23742 and RM23759 which are separated by approximately 486.5 kb. The results may facilitate map-based cloning of wsl1 and understanding of the molecular mechanism of the regulation of leaf-color by WSL1 in rice.

Key words: rice, white striped leaf mutant, genetic analysis, mapping

SHENG Zhong-hua, WEI Xiang-jin, SHAO Gao-neng, SONG Jian, LUO Ju, HU Pei-song, TANG Shao-qing2, CHEN Li-yun . Genetic Analysis and Molecular Mapping of Novel White Striped Leaf Mutant Gene in Rice[J]. RICE SCIENCE, 2013, 20(5): 336-342.

References

Beale S I. 2005. Green genes gleaned. Trends Plant Sci, 10: 309? 312.
Bevins M A, Madhavan S, Markwell J. 1993. Two sweet clover (Melilotus alba Desr.) mutants temperature sensitive for chlorophyll expression. Plant Physiol, 103: 1123–1231.
Chen T, Zhang Y D, Zhao L, Zhu Z, Lin J, Zhang S B, Wang C L. 2007. Physiological character and gene mapping in a new green-revertible albino mutant in rice. Genet Genom, 34: 331–338.
Delseny M, Salses J, Cooke R, Sallaud C, Regad F, Lagoda P, Guiderdoni E, Ventelon M, Brugidou C, Ghesquiere A. 2001. Rice genomics: Present and future. Plant Physiol Biochem, 39: 323–334.
Eckhardt U, Grimm B, H?rtensteiner S. 2004. Recent advances in chlorophyll biosynthesis and breakdown in higher plants. Plant Mol Biol, 56: 1–14.
Goh C H, Jung K H, Stephen K R, Martin R M, Alistair M H, Park Y, Suh K H, Gynheung A, Hong G N. 2004. Mitochondria provide the main source of cytosolic ATP for activation of outward-rectifying K+ channels in mesophyll protoplast of chlorophyll-deficient mutant rice (OsCHLH) seedlings. J Biol Chem, 279(8): 6874–6882.
Hiroki S, Kensuke K, Yuzuru T, Junshi Y, Naoki K, Shoshi K, Koh I. 2004. The virescent-2 mutation inhibits translation of plastid transcripts for the plastid genetic system at an early stage of chloroplast differentiation. Plant Cell Physiol, 45(8): 985–996.
Hoeberichts F A, Vaeck E, Kiddle G, Coppens E, Vande C B, Adamantidis A, Ormenese S, Foyer C H, Zabeau M, Inzé D. 2008. A temperature-sensitive mutation in the Arabidopsis thaliana phosphomannomutase gene disrupts protein glycosylation and triggers cell death. Biol Chem, 283: 5708?5718.
Huang X Q, Zhao H X, Dong C L, Sun Y Y, Wang P R, Deng X J. 2005. Chlorophyll-deficit rice mutants and their research advances in biology. Acta Bot Bor-Occ Sin, 25: 1685–1691. (in Chinese with English abstract)
Iwata N, Omura T, Satoh H. 1978. Linkage studies in rice (Oryza sativa L.) on some mutants for physiological leaf spots. J Fac Agric Kyushu Univ, 22: 243–251.
Kazumaru M, Yukihiro I, Akiko S, Nori K. 2003. OsHAP3 genes regulate chloroplast biogenesis in rice. Plant J, 36: 532–540.
Kodiveri M G, Kim E S, Cho H J, Chung Y Y. 2005. OsPPR1, a pentatricopeptide repeat protein of rice is essential for the chloroplast biogenesis. Plant Mol Biol, 58: 421–433.
Lan T, Liang K J, Chen Z W, Duan Y L, Wang J L, Ye N, Wu W R. 2007. Genetic analysis and gene mapping of cold-induced seedling chlorosis in rice. Hereditas, 29(9): 1121–1125. (in Chinese with English abstract)
Li X L, Sun X Q, Wang P R, Zhou H, Deng X J. 2010. Genetic analysis and gene mapping of a novel yellow-green leaf mutant in rice. Acta Agron Sin, 36(6): 1050–1054. (in Chinese with English abstract)
Li X Y, Wang C T, Li S W, He Y X, Chen S Q. 2012. The discovery of a high chlorophyll content gene in rice. Southwest Chin J Agric Sci, 15(4): 122–123. (in Chinese with English abstract)
Liu W Z, Fu Y P, Hu G C, Si H M, Zhu L, Wu C, Sun Z X. 2007. Identification and fine mapping of a thermo-sensitive chlorophyll deficient mutant in rice (Oryza sativa L.). Planta, 226: 785–795.
Liu Z L, Yuan S, Liu W J, Du J B, Tian W J, Luo M H, Lin H H. 2008. Mutation mechanism of chlorophyll-less barley mutant NYB. Photosynthetica, 46: 73–78.
Lu D H, Zong X F, Wang S G, Ling Y H, Sang X C, He G H. 2009. Characteristics of photosynthesis in two leaf color mutants of rice. Acta Agron Sin, 5(12): 2304–2308. (in Chinese with English abstract)
Pasini L, Bruschini S, Bertoli A, Mazza R, Fracheboud Y, Marocco A. 2005. Photosynthetic performance of cold-sensitive mutants of maize at low temperature. Physiol Plant, 124: 362–370.
Qian Q, Zhu X D, Zeng D L, Zhang X H, Yan X Q, Xiong Z M. 1996. The study of a novel white-green material controlled by cytoplasmic gene. Chin Seeds, (4): 11–12. (in Chinese)
Ryouhei M, Yutaka S, Yu M, Minoru N, Makoto K. 2009. Defect in non-yellow coloring 3, an a/b hydrolase-fold family protein, causes a stay-green phenotype during leaf senescence in rice. Plant J, 59: 940–952.
Su N, Hu M L, Wu D X, Wu F Q, Fei G L, Lan Y, Chen X L, Shu X L, Zhang X, Guo X P, Cheng Z J, Lei C L, Qi C K, Jiang L, Wang H Y, Wan J M. 2012. Disruption of a rice pentatricopeptide repeat protein causes a seedling-specific albino phenotype and its utilization to enhance seed purity in hybrid rice production. Plant Physiol, 159: 227–238.
Tang Y L, Huang J F, Wang R C. 2004. Change law of hyperspectral data with chlorophyll and carotenoid for rice at different developmental stages. Chin J Rice Sci, 18(1): 59–66. (in Chinese with English abstract)
Wang P R, Gao J X, Wan C M, Zhang F T, Xu Z J, Huang X Q, Sun X Q, Deng X J. 2010. Divinyl chlorophyll(ide) a can be converted to monovinyl chlorophyll(ide) a by a divinyl reductase in rice. Plant Physiol, 153: 994–1003.
Wu Z M, Zhang X, He B, Diao L P, Sheng S L, Wang J L, Guo X P, Su N, Wang L F, Jiang L, Wang C M, Zhai H Q, Wan J M. 2007. A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis. Plant Physiol, 145: 29–40.
Xu F H, Cheng Z J, Wang J L, Wu Z M, Sun W, Zhang X, Lei C L, Wang J, Wu P Q, Guo X P, Liu L L, Wan J M. 2010. Genetic analysis and fine-mapping of gws gene using green-white-stripe rice mutant. Acta Agron Sin, 36(5): 713–720. (in Chinese with English abstract)
Xu J J, Wang B H, Wu Y H, Du P N, Wang J, Wang M, Yi C D, Gu M H, Liang G H. 2010. Fine mapping and candidate gene analysis of ptgms2-1, the photoperiod-thermo-sensitive genic male sterile gene in rice (Oryza sativa L.). Theor Appl Genet, 122: 365–372.
Yoo S C, Cho S H, Hiroki S, Li J J, Kensuke K, Koh H J, Koh I, Paek N C. 2009. Rice virescent3 and stripe1 encoding the large and small subunits of ribonucleotide reductase are required for chloroplast biogenesis during early leaf development. Plant Physiol, 150: 388–401.
Yue B, Cai X W, Vick B, Hu J G. 2009. Genetic characterization and molecular mapping of a chlorophyll deficiency gene in sun-flower (Helianthus annuus). Plant Physiol, 166: 644–651.
Yue R Q, Wang X F, Chen J Y, Ma X X, Zhang H H, Mao C Z, Wu P. 2010. A rice stromal processing peptidase regulates chloroplast and root development. Plant Cell Physiol, 51(3): 475–485.
Zhang H T, Li J J, Yoo J H, Yoo S C, Cho S H, Koh H J, Seo H S, Paek N C. 2006. Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development. Plant Mol Biol, 62: 325–337.
Zhang L K, Li Z B, Liu H Y, Li R H, Chen M Y, Chen A G, Qian Y L, Hua Z T, Gao Y M, Zhu L H, Li Z K. 2010. Study on morphological structure and genetic mapping of two novel leaf color mutants in rice. Sci Agric Sin, 43(2): 223–229. (in Chinese with English abstract)
Zhu L, Liu W Z, Wu C, Luan W J, Fu Y P, Hu G H, Si H M, Sun Z X. 2007. Identification and fine mapping of a gene related to pale green leaf phenotype near the centromere region in rice (Oryza sativa). Rice Sci, 14(3): 172–180. (in Chinese with English abstract)
Zhu S Y, Luo T K, Zhang X L, Chen H Y, Tang Z, Liu Q. 2009. Comparison of 8 kinds of extraction methods on rice genomic DNA. J Anhui Agric Sci, 37(5): 1929–1931. (in Chinese with English abstract)

[1]	LI Qianlong, FENG Qi, WANG Heqin, KANG Yunhai, ZHANG Conghe, DU Ming, ZHANG Yunhu, WANG Hui, CHEN Jinjie, HAN Bin, FANG Yu, WANG Ahong. Genome-Wide Dissection of Quan 9311A Breeding Process and Application Advantages [J]. Rice Science, 2023, 30(6): 7-.
[2]	JI Dongling, XIAO Wenhui, SUN Zhiwei, LIU Lijun, GU Junfei, ZHANG Hao, Tom Matthew HARRISON, LIU Ke, WANG Zhiqin, WANG Weilu, YANG Jianchang. Translocation and Distribution of Carbon-Nitrogen in Relation to Rice Yield and Grain Quality as Affected by High Temperature at Early Panicle Initiation Stage [J]. Rice Science, 2023, 30(6): 12-.
[3]	Prathap V, Suresh KUMAR, Nand Lal MEENA, Chirag MAHESHWARI, Monika DALAL, Aruna TYAGI. Phosphorus Starvation Tolerance in Rice Through a Combined Physiological, Biochemical and Proteome Analysis [J]. Rice Science, 2023, 30(6): 8-.
[4]	Serena REGGI, Elisabetta ONELLI, Alessandra MOSCATELLI, Nadia STROPPA, Matteo Dell’ANNO, Kiril PERFANOV, Luciana ROSSI. Seed-Specific Expression of Apolipoprotein A-I_Milano Dimer in Rice Engineered Lines [J]. Rice Science, 2023, 30(6): 6-.
[5]	Sundus ZAFAR, XU Jianlong. Recent Advances to Enhance Nutritional Quality of Rice [J]. Rice Science, 2023, 30(6): 4-.
[6]	Kankunlanach KHAMPUANG, Nanthana CHAIWONG, Atilla YAZICI, Baris DEMIRER, Ismail CAKMAK, Chanakan PROM-U-THAI. Effect of Sulfur Fertilization on Productivity and Grain Zinc Yield of Rice Grown under Low and Adequate Soil Zinc Applications [J]. Rice Science, 2023, 30(6): 9-.
[7]	FAN Fengfeng, CAI Meng, LUO Xiong, LIU Manman, YUAN Huanran, CHENG Mingxing, Ayaz AHMAD, LI Nengwu, LI Shaoqing. Novel QTLs from Wild Rice Oryza longistaminata Confer Rice Strong Tolerance to High Temperature at Seedling Stage [J]. Rice Science, 2023, 30(6): 14-.
[8]	LIN Shaodan, YAO Yue, LI Jiayi, LI Xiaobin, MA Jie, WENG Haiyong, CHENG Zuxin, YE Dapeng. Application of UAV-Based Imaging and Deep Learning in Assessment of Rice Blast Resistance [J]. Rice Science, 2023, 30(6): 10-.
[9]	Md. Forshed DEWAN, Md. AHIDUZZAMAN, Md. Nahidul ISLAM, Habibul Bari SHOZIB. Potential Benefits of Bioactive Compounds of Traditional Rice Grown in South and South-East Asia: A Review [J]. Rice Science, 2023, 30(6): 5-.
[10]	Raja CHAKRABORTY, Pratap KALITA, Saikat SEN. Phenolic Profile, Antioxidant, Antihyperlipidemic and Cardiac Risk Preventive Effect of Chakhao Poireiton (A Pigmented Black Rice) in High-Fat High-Sugar induced Rats [J]. Rice Science, 2023, 30(6): 11-.
[11]	Nazaratul Ashifa Abdullah Salim, Norlida Mat Daud, Julieta Griboff, Abdul Rahim Harun. Elemental Assessments in Paddy Soil for Geographical Traceability of Rice from Peninsular Malaysia [J]. Rice Science, 2023, 30(5): 486-498.
[12]	Monica Ruffini Castiglione, Stefania Bottega, Carlo Sorce, Carmelina SpanÒ. Effects of Zinc Oxide Particles with Different Sizes on Root Development in Oryza sativa [J]. Rice Science, 2023, 30(5): 449-458.
[13]	Ammara Latif, Sun Ying, Pu Cuixia, Noman Ali. Rice Curled Its Leaves Either Adaxially or Abaxially to Combat Drought Stress [J]. Rice Science, 2023, 30(5): 405-416.
[14]	Liu Qiao, Qiu Linlin, Hua Yangguang, Li Jing, Pang Bo, Zhai Yufeng, Wang Dekai. LHD3 Encoding a J-Domain Protein Controls Heading Date in Rice [J]. Rice Science, 2023, 30(5): 437-448.
[15]	Lu Xuedan, Li Fan, Xiao Yunhua, Wang Feng, Zhang Guilian, Deng Huabing, Tang Wenbang. Grain Shape Genes: Shaping the Future of Rice Breeding [J]. Rice Science, 2023, 30(5): 379-404.